Version 2 2018-03-27, 10:49Version 2 2018-03-27, 10:49
Version 1 2017-10-05, 20:23Version 1 2017-10-05, 20:23
dataset
posted on 2018-03-27, 10:49authored byAngel MojarroAngel Mojarro, Julie Hachey, Gary Ruvkun, Maria T. Zuber, Christopher E. Carr
FastQ files with preserved headers containing channel information. The multiple files must first be combined into a single FastQ file prior to analysis (e.g., cat *.fastq > all_reads.fastq).
2 pg of B. subtilis DNA, quantified using ddPCR, prepared with 1000 ng of Lambda DNA using the Oxford Nanopore Technologies ligation sequencing kit - LSK-SQK108.
The library was then sequenced on a MinION Mark-1B sequencer and R9.4 flowcell for 48 hours and basecalled using ONTs MinKnow 1.5.18 live basecaller.