Supplementary material from "Are morphological characteristics of Parrotia (Hamamelidaceae) pollen species diagnostic?"

Parrotia persica is one of the most notable endemic relict tree species growing in the Hyrcanian forest at the southern Caspian Sea. The recent discovery of sibling species Parrotia subaequalis, occurring in the temperate for- ests of south-eastern China, offers the opportunity to compare their morphology and ecological preferences and to dig deeper into the paleophytogeographic history of the genus from a perspective. Since pollen morphology of these species would be essential to unravel the origin and evolution of these Arcto-Tertiary species, the present study aimed to investigate whether it is possible to segregate pollen from these two species. Therefore, a detailed combined light- and scanning electron microscopy-based pollen-analysis of each taxon was conducted, the pol- len was described, measured, and compared using statistical approaches and principal component analyses to es- tablish unbiased results. The correlation-based principal component analysis achieved for each species shows an overall good superposition of pollen grains measured in equatorial and polar views in the first principal plane, revealing that the P. persica pollen is morphometrically as homogeneous as that of P. subaequalis. Then, the sig- nificant difference, mainly driven by lumen density, has been highlighted between the two species. Ultimately, the cross-validation of the resulting two-species linear discriminants classifier shows that based upon this refer- ence dataset, (sub)fossil pollen grain can now be confidently assigned to either of the two species with an 85.8% correct-assignment rate. This opens new doors in the affiliation of fossil Parrotia pollen and suggests that previous pollen records need to be revised.