posted on 2021-05-11, 03:54authored bySunny Z. Wu, Daniel L. Roden, Ghamdan Al-Eryani, Nenad Bartonicek, Kate Harvey, Aurélie S. Cazet, Chia-Ling Chan, Simon Junankar, Mun N. Hui, Ewan A. Millar, Julia Beretov, Lisa Horvath, Anthony M. Joshua, Phillip Stricker, James S. Wilmott, Camelia Quek, Georgina V. Long, Richard A. Scolyer, Bertrand Z. Yeung, Davendra Segara, Cindy Mak, Sanjay Warrier, Joseph E. Powell, Sandra O’Toole, Elgene Lim, Alexander Swarbrick
Additional file 5. Differentially expressed genes for all cells across cryopreservation conditions. Differential gene expression was performed using the MAST method within Seurat v3 with the RNA assay and default parameters. Differential gene expression was performed using integrated data. Data from all conditions from each matched tumour datasets were down sampled to the same number of mapped sequencing reads using CellRanger. The number of cells in each condition was randomly down sampled to match the condition with the lowest number of cells.
Funding
National Breast Cancer Foundation The Sydney Breast Cancer Foundation National Health and Medical Research Council John and Deborah McMurtrie Australian Prostate Cancer Research Centre NSW Cancer Institute New South Wales