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A genetic screen suggests an alternative mechanism for inhibition of SecA by azide

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posted on 2017-08-06, 20:23 authored by Damon HuberDamon Huber, Mohammed Jamshad, Rachael Chandler, Mark Jeeves, Ashley Robinson, Farhana Alam, Tamar Cranford Smith, Anokhi Shah, Oliver Daubney, Karl A. Dunne, Naomi Nabi, Aadil Iqbal, Anna F. A. Peacock, Janet E. Lovett, Timothy Knowles, Ian Henderson
A high-density transposon library constructed in BW25113 was grown in the absence or presence of 0.25 mM or 0.5 mM sodium azide. In the case of the 0 mM and 0.25 mM samples, cells were grown until OD600 = 1.0. In the case of of the 0.5 mM sodium azide sample, the cells were grown to OD600 = 0.9, at which point the cells stopped growing exponentially. The location of the transposons after growth was determined using Illumina sequecing. Libraries were prepared using arbirary PCR and sequenced using single-end reads. Reads were then processed an aligned to the genome sequence for Escherichia coli K-12 strain W3110 (NCBI accession AP009048.1). Please see associated manuscript at BiorXiv for a more detailed description of the experiment.


BBSRC grant BB/L019434/1