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16S amplicon fusion primers and protocol for Illumina platform sequencing
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posted on 2013-10-29, 17:24
authored by
Hilary G. Morrison
Hilary G. Morrison
,
Sharon L. Grim
,
Joseph H. Vineis
,
Mitchell L. Sogin
<p>Description and sequences of fusion primers used to generate 16s amplicon libraries (variable domains v6 and v4-v5) for sequencing on Illumina platforms. PCR conditions and PCR clean up information also provided. </p>
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Categories
Plant cell and molecular biology
Animal cell and molecular biology
Ecology not elsewhere classified
Microbiology not elsewhere classified
Keywords
Illumina sequencing
microbial diversity
16s
universal
pcr
biodiversity
amplicon sequencing
Molecular Biology
Ecology
Microbiology
Licence
CC BY 4.0
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