16S_amplicon_Illumina_sequencing_methods.pdf (72.74 kB)

16S amplicon fusion primers and protocol for Illumina platform sequencing

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posted on 29.10.2013, 17:24 by Hilary G. Morrison, Sharon L. Grim, Joseph H. Vineis, Mitchell L. Sogin

Description and sequences of fusion primers used to generate 16s amplicon libraries (variable domains v6 and v4-v5) for sequencing on Illumina platforms.  PCR conditions and PCR clean up information also provided.  

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