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16S amplicon fusion primers and protocol for Illumina platform sequencing

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posted on 2013-10-29, 17:24 authored by Hilary G. MorrisonHilary G. Morrison, Sharon L. Grim, Joseph H. Vineis, Mitchell L. Sogin

Description and sequences of fusion primers used to generate 16s amplicon libraries (variable domains v6 and v4-v5) for sequencing on Illumina platforms. PCR conditions and PCR clean up information also provided.

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Illumina sequencing microbial diversity 16s universal pcr biodiversity amplicon sequencing Molecular Biology Ecology Microbiology

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