cSBL induced apoptotic changes in H2452 and MSTO cells.

Cells were treated with cSBL for the indicated times. (A, B) Rate of apoptosis as indicated by the percentage of annexin V-positive cells. (C) Nuclear fragmentation images were captured using 40X objective; a false-colored image of the nuclei (blue) is shown. White arrowheads indicate the cells with condensed or fragmented nuclei. (D, E) Fragmentation index indicating the degree of nuclear fragmentation; a higher index indicates greater occurrence of fragmentation, calculated using the High-Content Analysis System. All data are expressed as the mean ± SD of three independent experiments. The statistical significance of these experiments compared with the control is shown as follows: *P<0.05, **P<0.01, ***P<0.001.