Figure_5.tif (359.54 kB)
siRNA against CCT-eta decreases PDGF-induced fibroblast migration, whereas siRNA against CCT-beta does not.
figure
posted on 2013-02-21, 01:36 authored by Latha Satish, Sandra Johnson, James H-C. Wang, J. Christopher Post, Garth D. Ehrlich, Sandeep KathjuCells were incubated in the presence or absence of PDGF (200 nM) +/− siRNA against CCT-eta (a) or CCT-beta (b) in an in vitro wound healing assay. In all experiments a subunit -specific scrambled siRNA sequence was used as control. Cell motility is shown as a percentage of baseline migration in the absence of PDGF or siRNA exposure. As with EGF, active siRNA targeting CCT-eta inhibited basal and PDGF-induced motility, whereas CCT-beta siRNA and scrambled controls did not. Values are means ± SEM of six independent studies, each performed in triplicate. Statistical analyses were performed with Student's t test.