rEBNA-1 injected mice develop antibodies to EBNA-1 and dsDNA.

Mice were unimmunized or injected ip with rEBNA-1 emulsified in CFA or CFA alone and boosted with rEBNA-1 emulsified in IFA or IFA alone (arrows indicate week of initial injection and boosts). Mice were bled at weeks 1.5, 4, 6, 10, 12, 15, and 18 and sera tested by ELISA for antibody to EBNA-1 (A) and dsDNA (B). Results are the average of 5 mice in each group. Standard deviations are indicated. (C and D) Eight fold serial dilutions of week 12 sera from all 5 rEBNA-1 injected mice were tested by ELISA for antibody titers to EBNA-1 (C) and dsDNA (D). Dotted line represents 3 standard deviations above the mean absorption of sera from week 12 uninjected control mice. (E) Serum from a week 12 mouse injected with rEBNA-1 (left panel) or adjuvant only (right panel) was diluted 1∶50 and used to immunostain Crithidia luciliae slides. Immunostaining of the dsDNA in the kinetoplast of Crithidia luciliae is observed (left panel) as indicated by arrow. Results are representative of 3 rEBNA-1 and 3 adjuvant only, injected mice.