Fig_6.tif (703.15 kB)
qPCR validation of selected M1 and M2a,b,c-specific genes in human GAMs.
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posted on 2015-02-06, 03:26 authored by Frank Szulzewsky, Andreas Pelz, Xi Feng, Michael Synowitz, Darko Markovic, Thomas Langmann, Inge R. Holtman, Xi Wang, Bart J. L. Eggen, Hendrikus W. G. M. Boddeke, Dolores Hambardzumyan, Susanne A. Wolf, Helmut KettenmannWe determined the expression of the M1 (IL1RN and ISG20), M2a (CLEC7A), M2b (TGFBI), and M2c-specific (CXCR4) genes in CD11b+ and CD11b- cells isolated from human GBM (CD11b+ n = 13, CD11b- n = 5), control brain (CD11b+ n = 5, CD11b- n = 2) and blood monocyte samples (n = 2). Bar graphs illustrate the absolute number of transcripts normalized to 100,000 transcripts of ACTB. Analysis was done by students t test. Error bars indicate the SEM. *, p<0.05; **, p<0.01; ***, p<0.001
