pDmrt1-Cre;Vegfa^-/- and control ovary histology.

Hematoxylin-eosin staining of ovarian cross-sections of 200x control (A), 200x control (C), 200x KO (B), and 200x KO (D). The 200x images depict varied CL morphology between a control and KO. Some of the ovarian structures are labeled: corpus luteum (CL) and the stage of follicle (1–4). The number of primordial follicles (stage 0) and developing follicles (stages 1–5) were counted and compared between controls (n = 8) and KO (n = 9) (E). The number of CL per ovarian section was counted based on positive staining for CYP11A1 and compared between controls (n = 8) and KO (n = 6) (F). Results were significant when P < 0.05 and were represented with a *, and a † denoted data that tended to be significant (0.05 < P < 0.1).