hg-unc-25 is down regulated and fewer GABA-immunoreactive neurons are detected at the sedentary stages.

(A) Relative mean ± standard error expression of hg-unc-25, based on RT-qPCR. Data were normalized to J2 with hg-far-1 used as an internal control. Treatments with different letters are statistically different at α = 0.05 as determined by ANOVA. (B) Lateral left view cartoon (top) and representative fluorescent micrographs of GABA-immunostained J2 H. glycines sections (bottom). GABA immunostaining was conducted on bisected animals to facilitate penetration. The location of individual neurons was determined by their position relative to landmarks such as the esophagus (purple) and the primordial gonad (blue). GABA-immunoreactive neurons (green) are located surrounding the nerve ring, between the metacorpus and the esophageal glands, and along the VNC. (C) Ventral view fluorescent micrograph of anti-GABA stained H. glycines J4 female. Scale bars, 10 μm.