Netrin-1 mRNA and HCV NS5A are LARP1 interactants.

Quantification of ribosomal protein S18 (RPS18) (A) and Netrin-1 (B) mRNA by qPCR after immunoprecipitation using LARP1 antibody (data are represented as mean ± standard deviation, n = 3, Mann-Whitney test, p < 0.05). C. Huh-7.5 cells were cotransfected with a Gluc1-LARP1 plasmid and individual plasmids encoding Gluc2 fused to the indicated HCV proteins and subjected to luciferase assays. Y-axis represents normalized luminescence ratio (NLR). The dashed line represents background level measured using a 53-protein random reference set. Data represent means ± standard deviations (error bars) from three independent experiments in triplicates. The underlying data for panels in this figure can be found in S1 Data.