iNKT cell subtypes in the periphery.

(A–C) FACS profile of peripheral iNKT cells in B6 mice. α-GalCer/CD1d dimer⁺ TCRβ⁺iNKT cells (A) and iNKT subtypes based on the expression of CD44 and NK1.1 (B) or CD4 and IL-17RB (C) in spleen, liver, BM, lung, inguinal LN, and mesenteric LN from B6 or Il17rb^−/− mice. Numbers indicate percentage of total mononuclear cells (A) and iNKT cells (B, C). (D) Number of cells of each iNKT subtype based on the expression of IL-17RB and CD4 in thymus and periphery of B6 and BALB/c mice. Cell numbers were calculated based on the results from Figures 4A, 4C, S8A, and S8B. IL-17RB⁺iNKT cells were mainly localized in spleen, lung, inguinal LN, and mesenteric LN, whereas hardly any were observed in liver and BM. One representative experiment of three is shown.