<i>Digitor</i> mutant third instar larvae have a severely reduced brain size.

<p>(A) Images of dissected brains from wild type (WT) and <i>Digitor</i> mutant (<i>EP(3)3709/EP(3)3709</i>) third instar larvae at the same scale. (B) Comparison of the average area of the two optic lobes from 20 brains of wild type and <i>Digitor</i> mutant larvae with S.D., respectively. The difference in size was statistically significant (P value < 0.001, Student's two-tailed t-test). (C) Images of wild type and <i>Digitor</i> mutant third instar larvae side by side. (D) Comparison of the average from four determinations of the body weight of 22 wild type and <i>Digitor</i> mutant larvae with S.D., respectively. The difference in weight was not statistically significant (P value ≥ 0.8, Student's two-tailed t-test). (E) Acridine Orange labeling of brains from wild type and <i>Digitor</i> mutant larvae. (F) TUNEL labeling of brains from wild type and <i>Digitor</i> mutant larvae. (G) BrdU incorporation into brains from wild type and <i>Digitor</i> mutant larvae. (H) Anti-H3S10ph antibody labeling of brains from wild type and <i>Digitor</i> mutant larvae. (I) Comparison of the average fluorescence intensity of BrdU incorporation from the two optic lobes and thoracic segments from 10 brains of wild type and <i>Digitor</i> mutant larvae with S.D., respectively. The difference was statistically significant (P value < 0.01, Student's two-tailed t-test). (J) Comparison of the average fluorescence intensity of anti-H3S10ph antibody labeling from the two optic lobes and thoracic segments from 8 brains of wild type and <i>Digitor</i> mutant larvae with S.D., respectively. The difference was statistically significant (P value < 0.001, Student's two-tailed t-test).</p>