Digitor mutant third instar larvae have a severely reduced brain size.

(A) Images of dissected brains from wild type (WT) and Digitor mutant (EP(3)3709/EP(3)3709) third instar larvae at the same scale. (B) Comparison of the average area of the two optic lobes from 20 brains of wild type and Digitor mutant larvae with S.D., respectively. The difference in size was statistically significant (P value < 0.001, Student's two-tailed t-test). (C) Images of wild type and Digitor mutant third instar larvae side by side. (D) Comparison of the average from four determinations of the body weight of 22 wild type and Digitor mutant larvae with S.D., respectively. The difference in weight was not statistically significant (P value ≥ 0.8, Student's two-tailed t-test). (E) Acridine Orange labeling of brains from wild type and Digitor mutant larvae. (F) TUNEL labeling of brains from wild type and Digitor mutant larvae. (G) BrdU incorporation into brains from wild type and Digitor mutant larvae. (H) Anti-H3S10ph antibody labeling of brains from wild type and Digitor mutant larvae. (I) Comparison of the average fluorescence intensity of BrdU incorporation from the two optic lobes and thoracic segments from 10 brains of wild type and Digitor mutant larvae with S.D., respectively. The difference was statistically significant (P value < 0.01, Student's two-tailed t-test). (J) Comparison of the average fluorescence intensity of anti-H3S10ph antibody labeling from the two optic lobes and thoracic segments from 8 brains of wild type and Digitor mutant larvae with S.D., respectively. The difference was statistically significant (P value < 0.001, Student's two-tailed t-test).