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De Novo Synthesis of Basal Bacterial Cell Division Proteins FtsZ, FtsA, and ZipA Inside Giant Vesicles
journal contribution
posted on 2018-03-07, 00:00 authored by Takumi Furusato, Fumihiro Horie, Hideaki T. Matsubayashi, Kazuaki Amikura, Yutetsu Kuruma, Takuya UedaCell division is
the most dynamic event in the cell cycle. Recently,
efforts have been made to reconstruct it using the individual component
proteins to obtain a better understanding of the process of self-reproduction
of cells. However, such reconstruction studies are frequently hampered
by difficulties in preparing membrane-associated proteins. Here we
demonstrate a de novo synthesis approach based on
a cell-free translation system. Genes for fundamental cell division
proteins, FtsZ, FtsA, and ZipA, were expressed inside the lipid compartment
of giant vesicles (GVs). The synthesized proteins showed polymerization,
membrane localization, and eventually membrane deformation. Notably,
we found that this morphological change of the vesicle is forced by
only FtsZ and ZipA, which form clusters on the membrane at the vesicle
interior. Our cell-free approach provides a platform for studying
protein dynamics associated with lipid membrane and paves the way
to create a synthetic cell that undergoes self-reproduction.
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De Novo SynthesisFtsAlipid compartmentZipAmembrane deformationmembrane-associated proteinsGVCell Division Proteins FtsZgiant vesiclessynthesis approachtranslation systemGiant Vesicles Cell divisionform clusterscell cyclereconstruction studiescell division proteinsprotein dynamicslipid membranemembrane localizationself-reproductioncomponent proteins
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