<i>Candida albicans</i> biofilm formation on peptide functionalized polydimethylsiloxane

<div><p>In order to prevent biofilm formation by <i>Candida albicans</i>, several cationic peptides were covalently bound to polydimethylsiloxane (PDMS). The salivary peptide histatin 5 and two synthetic variants (Dhvar 4 and Dhvar 5) were used to prepare peptide functionalized PDMS using 4-azido-2,3,5,6-tetrafluoro-benzoic acid (AFB) as an interlinkage molecule. In addition, polylysine-, polyarginine-, and polyhistidine-PDMS surfaces were prepared. Dhvar 4 functionalized PDMS yielded the highest reduction of the number of <i>C. albicans</i> biofilm cells in the Modified Robbins Device. Amino acid analysis demonstrated that the amount of peptide immobilized on the modified disks was in the nanomole range. Poly-d-lysine PDMS, in particular the homopeptides with low molecular weight (2500 and 9600) showed the highest activity against <i>C. albicans</i> biofilms, with reductions of 93% and 91%, respectively. The results indicate that the reductions are peptide dependent.</p> </div>



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