<i>Bacillus</i> sp. JR3 esterase LipJ: A new mesophilic enzyme showing traces of a thermophilic past - Fig 6

<p>Zymogram analysis [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0181029#pone.0181029.ref031" target="_blank">31</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0181029#pone.0181029.ref032" target="_blank">32</a>] of cell extract and supernatant samples of cloned LipJ and <i>Bacillus</i> JR3, performed at 30°C (<b>A</b>) and 60°C (<b>B</b>). Lane 1: crude cell extract of <i>E</i>.<i>coli</i> expressing LipJ; Lane 2: supernatant of <i>B</i>. <i>cereus</i> 131, used for comparison; Lanes 3–4: soluble (3) and insoluble (4) cell extract of <i>E</i>.<i>coli</i> without the <i>lipJ</i> insert, used as a negative control; Lanes 5–6: concentrated supernatant (5) and crude cell extract (6) of <i>Bacillus</i> strain JR3.</p>