B. pertussis growth and production of ammonia in media with different glutamate:fumarate ratios.
A) The growth of B. pertussis was monitored by measuring the increase in OD600 of cultures over time. The average OD of 10 replicate cultures is shown. B) Ammonia production was measured in the supernatants of 5 cultures for each medium at the end of growth. The average for each medium type is shown.