Fig_5.tif (9.36 MB)

cGMP/PKG signal transduction is involved in the effect of NO on HaCaT cell migration.

figure

posted on 2015-03-23, 05:57 authored by Rixing Zhan, Shiwei Yang, Weifeng He, Fan Wang, Jianglin Tan, Junyi Zhou, Sisi Yang, Zhihui Yao, Jun Wu, Gaoxing Luo

(a) Treatment with a cGMP agonist (8-Br-cGMP, 1 μM) or PKG agonist (8-pCPT-cGMP, 1 μM) alone. The cells were pretreated with a cGMP inhibitor (8-Br-cAMP, 1 μM), PKG inhibitor (8-CPT-cAMP, 1 μM), Rho-specific inhibitor, (Rhosin, 30 μM), CDC42 inhibitor (ZCL278, 30 μM) or Rac1 inhibitor (Z62954982, 50 μM) for 2 hours, followed by the addition of SNP at a final concentration of 10 μM and incubation for another 24 hours. (b) The results are expressed as the means ± SD of 3 experiments.