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XMetS potentiates insulin-dependent Akt and Erk phosphorylation.

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posted on 2014-02-12, 03:22 authored by John A. Corbin, Vinay Bhaskar, Ira D. Goldfine, Daniel H. Bedinger, Angela Lau, Kristen Michelson, Lisa M. Gross, Betty A. Maddux, Hua F. Kuan, Catarina Tran, Llewelyn Lao, Masahisa Handa, Susan R. Watson, Ajay J. Narasimha, Shirley Zhu, Raphael Levy, Lynn Webster, Sujeewa D. Wijesuriya, Naichi Liu, Xiaorong Wu, David Chemla-Vogel, Steve R. Lee, Steve Wong, Diane Wilcock, Mark L. White

A. CHO-hINSR cells were preincubated for 10 minutes at 37°C with either the IgG2 form of XMetS, the Fab form of XMetS, or control antibody followed by a 10 minute incubation with increasing concentrations of insulin. Phosphorylation of Akt was then determined. B. CHO-hINSR cells were preincubated for 10 minutes at 37°C with a wide range of XMetS concentrations followed by a 10 minute incubation with increasing concentrations of insulin. Schild analysis [30] of these data was carried out using a curve fit algorithm (Prism, allosteric EC₅₀ shift). C. EC₅₀ values were determined from each curve fit (of the data presented in B) and are plotted as a function of XMetS concentration. D. CHO-hINSR cells were preincubated for 10 minutes at 37°C with either XMetS or control antibody followed by a 10 minute incubation with increasing concentrations of insulin and phosphorylation of Erk was then determined. The mean of duplicate determinations are shown.