Figure_4.tif (1.54 MB)
XLHMGA2 and XHMG-AT-hook1 DNA-binding properties.
figure
posted on 2013-07-25, 01:44 authored by Simone Macrì, Riccardo Sgarra, Gloria Ros, Elisa Maurizio, Salvina Zammitti, Ornella Milani, Marco Onorati, Robert Vignali, Guidalberto Manfioletti(A) Electrophoretic mobility shift assay performed with in vitro transcribed and translated (IVT) HA-tagged XLHMGA2βa (HA-XLA2ßa) and XHMG-AT-hook1 (HA–XATH1) proteins. Two different DNA probes were used: upper panel, E3 (0.1 pmoles); lower panel HCRII (0.1 pmoles); EMSAs were performed incubating 2, 4, and 6 µL of IVT proteins. (B) Western blot analysis of IVT proteins is shown (red ponceau stained membrane (left) and α-HA antibody recognition (right) to assess the production of the XLHMGA2βa and XHMG-AT-hook1 proteins.
