Wild type Shigella induce TEER decrease in Caco2 cell monolayers independent of MOIs.

A. TEER responses to wild-type S. flexneri 2a applied at three different inocula. B. TEER responses to S. flexneri 2a heat-killed bacteria and culture supernatants (Cond. Media). C. TEER responses to wild-type S. dysenteriae 1 applied at three different inocula. D. TEER responses to S. dysenteriae 1 heat-killed bacteria and culture supernatants. Data are expressed as means ± SEM for triplicate samples for all conditions tested. * and # indicate statistically significant differences vs. t = 0 and vs. the uninfected control at the same time point, respectively; p<0.05 (ANOVA). These results are representative of 3 experiments with similar results.