Western blotting analysis of BMP2 protein levels induced by uniaxial cyclic stretch in different groups.

(WT: wide-type) (A) BMP2 protein can be detected in all the groups and the expression levels were up regulated after mechanical stress was applied. (B) Quantification of BMP2 protein expression in all the groups. The expression of BMP2 protein in the C3H10T1/2 cells transfected by pcDNA3.1-BMP2 (rs2273073 (T/G)) and pcDNA3.1-BMP2 (rs2273073 (T/G), rs235768 (A/T)) were significantly higher than the other stretched groups (P<0.01) and the corresponding static groups (P<0.05). **P<0.01 versus un-transfected in the static condition; ##P<0.01 versus un-transfected in the stretched condition; &&P<0.01 versus the other stretched groups; $ P<0.05 versus the corresponding static groups.