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Western blot assays of expression of P35 and OP-IAP protein in T3 transgenic cotton lines.

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posted on 2013-02-20, 22:03 authored by Juan Tian, Xueyan Zhang, Benguo Liang, Shanwei Li, Zhixia Wu, Qianhua Wang, Chunxu Leng, Jiangli Dong, Tao Wang

The total soluble protein extracted from the young leaves of the T3 transgenic lines and non-transformed negative controls. (A) P35 expression. (B) OP-IAP expression. Lane 1 the negative control plant; lane 2, 3, 4 the three T3 transgenic lines (KB1, KB2, KB3); lane M molecular mass marker is PageRuler TM prestained protein ladder (MBI Fermentas, Maryland, USA).

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