Western blot analysis of p53 and the CDK inhibitor p27<sup>Kip1</sup> proteins.

<p>Total protein analysis confirmed no significant changes in the p53 expression after costunolide or dehydrocostus lactone treatment. In dehydrocostus lactone treated TE-671 and SW-872 cells, the expression of the CDK inhibitor p27<sup>Kip1</sup> was reduced. β-actin was used as loading control. Data shown are representative from at least three independent experiments.</p>