Western blot analysis of p53 and the CDK inhibitor p27Kip1 proteins.

Total protein analysis confirmed no significant changes in the p53 expression after costunolide or dehydrocostus lactone treatment. In dehydrocostus lactone treated TE-671 and SW-872 cells, the expression of the CDK inhibitor p27Kip1 was reduced. β-actin was used as loading control. Data shown are representative from at least three independent experiments.