Figure_6.tif (6.4 MB)
Warfarin affects carboxylation of MGP in atherosclerotic plaque.
figure
posted on 2013-02-20, 00:13 authored by Leon J. Schurgers, Ivo A. Joosen, Eduard M. Laufer, Martijn L. L. Chatrou, Marjolein Herfs, Mark H. M. Winkens, Ralf Westenfeld, Verena Veulemans, Thilo Krueger, Catherine M. Shanahan, Willi Jahnen-Dechent, Erik Biessen, Jagat Narula, Cees Vermeer, Leonard Hofstra, Chris P. ReutelingspergerTo confirm action of warfarin locally in the plaque we stained plaques for ucMGP and cMGP. UcMGP is the result of vitamin K-deficiency. Within one week of warfarin treatment the amount of cMGP (A,E) decreased significantly and decreased even further after 4 weeks of warfarin treatment. The decrease in cMGP was accompanied by an increase in ucMGP (B,F). Immunohistochemistry for cMGP and ucMGP (E,F) showed increased amounts of ucMGP compared with cMGP in apoE−/− mice on warfarin, whereas apoE−/− mice on control diet had predominantly cMGP (C,D). cMGP indicates carboxylated MGP; ucMGP, uncarboxylated MGP. Statistically significant differences were determined by the Kruskal Wallis test. *P<0.05, **P<0.01. i, intima; m, media; l, lumen.
