Verrucarin A inhibits SRC-3 downstream target gene (MMP2 and MMP13) expression.

<p>(A–B) Luciferase assays were performed in HeLa cells transiently transfected with MMP2-Luc, MMP13-Luc, and pCR3.1-SRC-3 expression vectors before incubation with verrucarin A at different concentrations (0, 2, 5, and 10 nM) for 24 h. (C) H1299 cells were treated with verrucarin A at different concentrations (0, 10, and 20 nM) for 24 h, then real-time PCR was performed to quantitate SRC-1, SRC-2, SRC-3, MMP2, and MMP13 mRNA expression.</p>