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Validation of RNA-seq data by using real-time polymerase chain reaction (PCR).

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posted on 2014-10-03, 03:05 authored by Jinwei Hu, Feng You, Qian Wang, Shenda Weng, Hui Liu, Lijuan Wang, Pei-Jun Zhang, Xungang Tan

The expression of trypsinogen2, chymotrypsinogen2, trypsinogen1, CYP51, PDE9A, trypsinogen3, LA4, EGFRK8 was detected by RNA-seq (blue column) and qPCR (red column). X-axis, group name; Y-axis, fold change in gene expression.

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