VEGF repression effect on ovulation, pregnancy and implantation rate.

<p>(<b>A</b>) Reversible repression system of VEGF gene expression. (<b>B</b>) Relative VEGF mRNA levels by qPCR (n = 5). (<b>C</b>, <b>D</b>) Protein expression by Western blot analysis and the quantification (n = 3). (<b>E</b>) Embryo collection at E3.5 (n = 45). (<b>F</b>) Embryo dissection at E9.5 (n = 11). (<b>G</b>) Embryo transfer experiment (n = 22). Dox+ represented mouse chow with 200 mg/kg doxycycline. Dox<b>−</b> represented regular mouse chow. Data are presented as mean ± SEM. *P<0.05, **P<0.005, ***P<0.001.</p>