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VEGF repression effect on ovulation, pregnancy and implantation rate.

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posted on 2013-03-01, 12:49 authored by Yan Ji, Xiaodan Lu, Qingping Zhong, Peng Liu, Yao An, Yuntao Zhang, Shujie Zhang, Ruirui Jia, Isaias G. Tesfamariam, Abraha G. Kahsay, Luqing Zhang, Wensheng Zhu, Yaowu Zheng

(A) Reversible repression system of VEGF gene expression. (B) Relative VEGF mRNA levels by qPCR (n = 5). (C, D) Protein expression by Western blot analysis and the quantification (n = 3). (E) Embryo collection at E3.5 (n = 45). (F) Embryo dissection at E9.5 (n = 11). (G) Embryo transfer experiment (n = 22). Dox+ represented mouse chow with 200 mg/kg doxycycline. Dox represented regular mouse chow. Data are presented as mean ± SEM. *P<0.05, **P<0.005, ***P<0.001.

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