Unilateral ureteral obstruction (UUO)-induced kidney fibrosis was attenuated in Akt2 knockout (KO) mice.

(A) Sections from control and obstructed kidneys from WT and Akt2 KO mice were stained with Masson's-modified trichrome. The blue color shows extracellular collagen deposition. (B) Immunohistochemistry for Fibronectin in unobstructed and obstructed kidneys from WT and Akt2 KO mice. Magnification: ×400. (C and D) Western blot analysis of Fibronectin and Collagen I protein expression in obstructed and unobstructed kidneys of WT and Akt2 KO mice. GAPDH was used as internal loading control. Band intensities were calculated using Scion Image software. Values are means ± SD (n = 6). ^##P<0.01 compared to the. nonobstructive kidney from WT mice; ^*P<0.05, ^**P<0.01 compared to the obstructive kidney from WT mice.