Unilateral ureteral obstruction (UUO) activated Akt and TGF-β1 signaling in obstructed kidneys.

(A) Immunohistochemical staining of p-Akt(Ser473) in wide-type (WT) mice after surgery for 5 days. Magnification: ×400. (B) Time course for the effects of UUO on expression of p-Akt(Ser473), Akt1 and Akt2, respectively by western blot analysis. (C and D) Time course for the effects of UUO on expression of TGFβ1 and p-Smad3 in mouse kidneys by western blot analysis. GAPDH was used as internal loading control. Band intensities were calculated using Scion Image software. Data are presented as means ± SD (n = 6). #P<0.05, ##P<0.01 compared to the control group.