Unfertilized, mature B. malayi oocytes contain a polar MTOC.

Mature oocytes (A,C) in meiosis I with bivalents associated with the anterior cortex, and embryos in first zygotic metaphase (B,D) are stained for DNA (blue), α-tubulin (red), and either the PCM marker Zyg-9 (A, B) or γ-tubulin (C,D) (green). In the oocyte, Wolbachia are associated with both poles and distributed in the cytoplasm. By the first zygotic division, Wolbachia are associated with the posterior pole. Scale bar = 5 µm.