Treg depletion allows high dose CP infection induced antigen sensitization.

<p>FoxP3-DTR tg mice received either CP+diphtheria toxin (DT) (n = 9), CP+PBS (n = 9), PBS+DT (n = 7), or PBS+PBS (n = 7), and were sensitized and challenged with HSA as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020784#pone-0020784-g001" target="_blank">Figure 1E</a>. <b>A:</b> Representative flow cytometric analysis of Treg depletion the day after DT injection. <b>B:</b> Representative staining of lung eosinophils in CP infected and DT treated mice. <b>C:</b> Representative staining of lung goblet cells in CP infected and DT treated mice. <b>D:</b> Total numbers of eosinophils were related to the total area of the section. <b>E:</b> Total numbers of goblet cells were related to the total length of bronchial basal membrane in the section. <b>F:</b> Mediastinal lymph node cells were restimulated with HSA after sensitization. Supernatants were measured for IL-5 by ELISA. <b>G:</b> HSA-specific IgE, IgG1, and IgG2a titers. *p≤0.05, **p≤0.01, ***p≤0.001.</p>