Transduction in the hippocampus and cerebellum as indicated by GFP expression.

Low magnification images show widespread GFP protein throughout the hippocampus (A) and cerebellar cortex (C). GFP protein was also observed in the contralateral hippocampus (B). Double immunostaining for GFP (green) and NeuN (red) revealed that neurons were specifically transduced (D–O). Merged images (F, I, L, O) also show Hoechst counterstaining. Neurons in many hippocampal sub-regions were transduced including Amon’s horn (D–F) and dentate gyrus (G–I). GFP was observed in Purkinje cells and other neurons in the cerebellum (M–O). Scale bar  = 500 µm (A–C) and 50 µm (D–O).