Tissue-specific patterns of expression for candidate pheromones assayed via reverse transcription PCR.

Expression patterns for SPα, SPα′ and SPβ transcripts and actin were assayed from three females and three males. Gels (A) and (B) correspond to SPα-α′ and SPβ, respectively. M, weight marker; O, ovary; MNG, main nidamental gland; OG, oviduct gland; G, gills; NS, nervous system; T, testis; SG, secondary glands; P, Penis; Ctrl−, negative control (no template added to PCR mix); Ctrl+, positive control (the template was a plasmid containing the insert of interest).