Tissue-specific patterns of expression for candidate pheromones assayed via reverse transcription PCR.

<p>Expression patterns for SPα, SPα′ and SPβ transcripts and actin were assayed from three females and three males. Gels (A) and (B) correspond to SPα-α′ and SPβ, respectively. M, weight marker; O, ovary; MNG, main nidamental gland; OG, oviduct gland; G, gills; NS, nervous system; T, testis; SG, secondary glands; P, Penis; Ctrl−, negative control (no template added to PCR mix); Ctrl+, positive control (the template was a plasmid containing the insert of interest).</p>