Timeline of anatabine treatment and behavior tests performed in Tg PS1/APPswe mice and their control wild-type littermate.

Oral anatabine treatment with 10mg/Kg/day or 20mg/Kg/Day was started in 10 months old Tg PS1/APPswe mice and control wild-type littermates and lasted for 6 and half months. During the course of treatment, a battery of behavior tasks was performed at the time points indicated above. Possible effects of the anatabine treatment on anxiety in mice were evaluated using the elevated plus maze; 0.5 month and 2.5 months after the initiation of the anatabine treatment (age of mice 10.5 and 12.5 months respectively). To evaluate the effects of anatabine on social interaction and social memory, the 3-chamber test was used 6 months after the treatment initiation (age of mice 16 months). At the end of study mice were humanely euthanized at the age of 16.5 months for pathological evaluations.