Time course of glomerular expression of Col4, SMA, pSrc and pSmad1 in Thy1 GN.

<p>(A, B) Staining scores per glomerular cross-section for Col4 (*<i>P</i><0.001, **<i>P</i><0.001) and SMA (*<i>P</i><0.001 and **<i>P</i> = 0.009) were calculated. Data represent mean values ± S.D. of at least three independent experiments; n = 6 for each experimental group. (C, D) Quantification of glomerular pSrc and pSmad1 by optical densitometry. The pSrc-positive nuclei and pSmad1-positive nuclei were counted in 10 consecutive fields in each specimen and normalized by the number of DAPI-positive nuclei. *<i>P</i><0.001, **<i>P</i><0.001. (E) Western blot for the glomerular lysates from each group. Data represent mean values ± S.D. of at least three independent experiments; n = 6 for each experimental group.</p>