Time course of glomerular expression of Col4, SMA, pSrc and pSmad1 in Thy1 GN.

(A, B) Staining scores per glomerular cross-section for Col4 (*P<0.001, **P<0.001) and SMA (*P<0.001 and **P = 0.009) were calculated. Data represent mean values ± S.D. of at least three independent experiments; n = 6 for each experimental group. (C, D) Quantification of glomerular pSrc and pSmad1 by optical densitometry. The pSrc-positive nuclei and pSmad1-positive nuclei were counted in 10 consecutive fields in each specimen and normalized by the number of DAPI-positive nuclei. *P<0.001, **P<0.001. (E) Western blot for the glomerular lysates from each group. Data represent mean values ± S.D. of at least three independent experiments; n = 6 for each experimental group.