Time Course of Actin Filament Formation and Capsid Assembly in the Nucleus

(A) SCG neurons were infected with PRV expressing GFP-VP26 at fixed times shown. The inset at 6 hpi is an enlargement of the nucleus from the cell on the right, which has small nuclear actin filaments. The brightness of the inset image has been enhanced in order to more clearly visualize the filaments. The arrowheads indicate actin filaments that appear to emanate from the nuclear envelope at 9 hpi. A single focal plane through the nucleus is shown. Scale bar = 20 μm.

(B) Chart showing the relative formation of nuclear actin filaments, the presence of GFP-VP26 fluorescence, and emergence of GFP-VP26 foci over the course of infection.