Threonine phosphorylation at JM regions influences the monomer–dimer equilibrium of EGF-bound EGFR.

<p>(A-D) Time traces of the diffusion coefficient of the EGFR with JM region threonine residue mutations; phosphodeficient mutant, EGFR<sup>T654/669A</sup> (A), three phosphomimic mutants, EGFR<sup>T654/669E</sup> (B), EGFR<sup>T654E</sup> (C), and EGFR<sup>T669E</sup> (D). (E) EGF (Alexa647-labeled, 0.17 μM; magenta) binding to EGFR<sup>T654/669E</sup> (green) expressing cell. The right panel shows the merged image. Scale bars represent 10 μm.</p>