Thermal unfolding parameters of WT- and Opti-Pgp.

a<p>Temperatures corresponding to the two maxima of the unfolding profiles seen in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0022577#pone-0022577-g006" target="_blank">Fig. 6</a>.</p>b<p>Number of independent experiments.</p>c<p>Averages ± range are given.</p>d<p>Experiments were routinely conducted in 20 mM HEPES, pH 7.6, 50 mM NaCl, 10% glycerol, 0.1% DDM and 5.5 mM DTT. Four experiments were conducted in buffers containing 40 mM imidazole, and three experiments were conducted with reduced glycerol (5% instead of 10% glycerol); no significant differences in the T<sub>m</sub> or ΔH<sub>cal</sub> were observed under those conditions.</p>