The short form of SIRT3 was downregulated in the murine hypertrophic heart.

(A and B) The velocity of the binding aorta was measured by echocardiology. The velocity was significantly elevated one week after TAC. (C) The degree of hypertrophy was assessed by the ratio of the heart weight to the body weight. Mice subjected to TAC produced nearly 60% cardiac hypertrophy at six weeks. (D) The subcellular fractionation from the hearts of WT mice after sham or TAC was analyzed by western blot analysis with antibodies specific for both forms of SIRT3, and β-actin was used as a reference. (E and F) Quantification of both forms of SIRT3 was determined by densitometry analysis, and β-actin was used as a reference. The densitometry value of each band was determined with the ImageJ software. The data are presented as the means ± SEM of three independent experiments. *P<0.05, **P<0.01.