The proliferation curve of A549, H460 and 293T cell lines after pargyline treatment and transfection of Flag-LSD1 or LSD1 siRNA.

The series of curves represent cell proliferation at 0 h to 48 h after exposure to different concentrations of pargyline treatment and transfection with the different plasmids. (A) The LSD1 inhibitor, pargyline (from top to bottom: 1mM, 2mM, 3mM and 4mM), and LSD1 siRNA inhibited proliferation of the A549 cell line. The A549 cell proliferation was significantly decreased in the different groups, as compared to the controls (*P<0.05). (B) Pargyline inhibited H460 cell line survival. Moreover, a stronger inhibitory effect was observed in the H460 cell line, but there were no significant differences between the two cell lines. The H460 cell proliferation was significantly decreased in the different concentration groups, as compared to the controls (*P<0.05). (C) Pargyline inhibited the survival of the 293T cell line. (D) The proliferation curve after transfection of Flag-LSD1 plasmid into the A549 cell and transfection of LSD1 siRNA plasmid into the H460 cell. In A549 cells, the proliferation was significantly higher after transfection of the Flag-LSD1 plasmid, as compared to the pCMV5-transfected control (*P<0.05). In H460 cells, the proliferation was significantly lower after transfection of LSD1 the siRNA plasmid, as compared to the cells transfected with siRNA control plasmid (*P<0.05).