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The pMCAO model produced cortical infarcts where core, periinfarct, and contralateral areas were isolated for RNA extraction.

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posted on 2012-12-17, 01:12 authored by Jaime Ramos-Cejudo, María Gutiérrez-Fernández, Berta Rodríguez-Frutos, Mercedes Expósito Alcaide, Fátima Sánchez-Cabo, Ana Dopazo, Exuperio Díez–Tejedor

Sprague-Dawley rats were subjected to permanent middle cerebral artery occlusion with a 9-0 suture for 24 h or 3 d (a) Schematic drawing of brain areas used for tissue sampling and RNA extraction. (b) Panel shows workflow and study groups used (n = 12 animals/condition). * = 4 RNA pools/condition (1 pool = 3 animals = 1 microarray). (CT: Contralateral cortex; PI: Periinfarct; C: Core).

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