The long lasting effects of corticosterone treatment on AHPs and action potential firing of hippocampal CA1 pyramidal neurons.
A: schematic depiction of the experimental protocol. B: locations of the recorded neurons on a pictorial coronal mouse brain section. C: representative AHP responses from vehicle and corticosterone groups overlaid with the fit. Light gray: raw trace, thick dark gray: fit, dotted line: mAHP decay, thin black line: sAHP decay. Inset shows the current protocol used to evoke the AHPs. D: traces of action potentials from neurons representative of the vehicle and corticosterone groups. Marking on the trace indicate the positions of spike amplitude, half-width and threshold measurements. E: mAHP peak amplitude averaged per group and per current step. F: averaged values of sAHP peak amplitude for each current injection step. G: sAHP peak amplitude is correlated with total number of action potentials per current step. RVeh/RCort: Pearson’s correlation coefficient for vehicle and corticosterone treated groups. *: P<0.05; **: P<0.0001. H: accommodation index (% ratio of the interspike intervals of the last two spikes to the first two spikes) from vehicle and corticosterone groups. PInt: a significant interaction effect of corticosterone. Number within brackets in the legends indicates number of neurons.
