The functional influence of the RNA transcript complementary to pri-miR-122 for the expression levels of pri-miR-122 and miR-122.

(A) RNA extracted from Huh-7 cells infected with various lenti-ADARs was prepared for assessment of the levels of endogenous miR-122, with that of si-GFP infected cells (set as 1). (B) RNA extracted from HepG2 cells transfected with either the wild-type or the mutant pri-miR-122 containing A-to-G at position −7 was processed for qRT–PCR. (C) The protein lysates extracted from Huh-7 (left) and HepG2 (right) cells infected with various lentiviruses were processed for the Western blotting analysis. (D) RNA extracted from Huh-7 cells infected with either lenti-si-GFP or lenti-si-AS-122, which targets the RNA transcript complementary to pri-miR-122, was processed for qRT–PCR for the antisense transcript (comp-AS-RNA) (left), the pri-miR-122 (middle), and miR-122 (right). (E) The HepG2 cells transfected with the control vector or the plasmid construct expressing comp-AS-RNA were processed for qRT–PCR, for examining the levels comp-AS-RNA (left), pri-miR-122 (middle), and miR-122 (right). The results were shown as the average of three experiments (mean ± SD), and the P values were calculated by t test (*P<0.05; ***P<0.001).