The effects of silencing Pik3ip1 on the PI3K/AKT/mTOR pathway.

NRCMs were transfected with the indicated siRNA for 24 h and subsequently serum-starved for 24 h. (A) PI3K activity (p110α) in the siNegative and siPik3ip1-transfected NRCMs. Western blot analyses of samples from siNegative and siPik3ip1-transfected NRCMs were performed after 48 h. (B–E) Representative immunoblot images of phosphorylated protein and total protein (upper), and the ratio of phosphorylated protein/total protein (bottom) for (B) AKT, (C) mTOR, (D) p70s6k, and (E) eEF2. (F) ERK 1/2 (n = 3, * p < 0.05 and ** p < 0.01, t test)