The effects of MBL on the expressions of apoptosis regulators in monocytes.
The effects of MBL on the mRNA expression of caspase-3, Fas, FasL, Bax, and Bcl-2 as shown by real-time RT-PCR (A and B). Monocytes were treated with vehicle, HSA (20 μg/ml) and MBL (20 μg/ml) for 48 h (A). U937 cells were treated with vehicle, HSA (20 μg/ml) and MBL (20 μg/ml) for 72 h (B). MBL induced caspase-3 activity (C). U937 cells were treated with 20 μg/ml of MBL or vehicle for 4 h and 6 h. The activity of caspase-3 was determined by FCM. The effects of MBL on caspase-3, PARP, Fas and FasL protein expression, as determined by Western immunoblotting (D). U937 cells were treated with MBL at 20 μg/ml or vehicle for 72 h. Caspase-3 activation and PARP cleavage were detected by Western blotting. GAPDH was used as the internal control. Data were mean ± S.D. of three independent experiments. Levels of statistical significance were: * p<0.05, ** p<0.01, *** p<0.001 as compared control cultures.