The effects of CO on STAT3 glutathionylation.

A. and B. ECs were treated with 100 µM BioGEE for one hour and loaded with TCDC or MC for the indicated times to induce protein glutathionylation. C. and D. ECs were exposed to TCDC or MC for the indicated periods. S-glutathionylated STAT3 was immunoprecipitated and detected using an antibody against protein-SSG. The quantification of the band intensities was normalized to the control values. Results are presented as the mean ± SEM (n = 3). *P<0.05 compared with untreated ECs.