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The effect of recombinant ToxA and ToxB administration on shrimp.
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posted on 2015-05-27, 04:03 authored by Ratchanok Sirikharin, Suparat Taengchaiyaphum, Piyachat Sanguanrut, Thanh Duong Chi, Rapeepat Mavichak, Porranee Proespraiwong, Bunlung Nuangsaeng, Siripong Thitamadee, Timothy W. Flegel, Kallaya Sritunyalucksana(A) Graph of cumulative mortality up to 48 h from single and mixed toxins at various concentrations. The graph contains results from two experiments, each with a BSA control (10 ug/g shrimp) (SD = 0) and with co-administration of 10 ug/g each of ToxA+ToxB (SD bars). The other treatments were not duplicated (no SD bars). (B) Graph of mortality at 24 h post administration versus mixed toxin concentration, yielding a linear regression line and rough LD50 for the mixed toxins of approximately 6 ug/g of each.
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parahaemolyticus M 0605similarity51 VPAHPNDcontig 034 GenBank accessionhepatopancreatic necrosis diseasegavage bioassaysmarker levelsToxA geneammonium sulfate precipitationcause AHPND pathologybroth cultureVibrio parahaemolyticus IsolatesMASCOT analysishepatopancreatic tubule epithelial cellsVibrio parahaemolyticusJALLtubule epithelial cellsPCR detectionnematode bacterium Photorhabdus luminescensgavage assaysshrimp pondsPCR methodbroth cultures16 kDavivo tests50 kDashrimp stomachmass spectrometry analysisPhotorhabdus insectprotein fractionshistological analysisCause Acute Hepatopancreatic Necrosis Diseaseinsecticidal toxins
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